Improving soleus muscle function in male Wistar rats by increasing calcitonin gene-dependent peptide (CGRP) during a period of moderate-intensity continuous rehabilitation training

Document Type : Research Paper

Authors

1 Department of Physical Education, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

2 Department of Physical Education, Razi University, Kermanshah, Iran

Abstract

Background and aim: Decreased physical activity is associated with poor neuromuscular function for daily activities. Laboratory studies indicate the effect of calcitonin gene-related peptide (CGRP) on neuromuscular activity. The aim of this study was to investigate the effect of a course of moderate-intensity continuous rehabilitation exercises (MCT) on CGRP expression in skeletal muscle of male Wistar rats.
Materials and Methods: For this purpose, 12 male Wistar rats weighing 200-220 g, with a sleep cycle of 12.12, free access to food and water and ambient temperature of 25±2 ° C were randomly divided into two groups of exercise and control. The training group performed 6 sessions (5 sessions per week) of continuous training with moderate intensity. The conditions of the control group were similar to the training group but they did not exercise. Finally, 48 hours after the last training session, rats were extracted by standard anesthesia, victim and horseshoe muscle method of all mice, and then Western blot method was used to determine the expression of CGRP protein. Significance level was considered p> 0.05.
Results: Statistical findings from the output of independent t-test for CGRP receptor protein expression values showed that the implementation of six weeks of MCT training protocol significantly increased CGRP protein expression in the MCT group compared with the control group (P = 0.048).
Conclusion: These results indicate that the MCT rehabilitation protocol caused that muscle function during endurance activities could be improved by increasing CGRP protein expression.

Keywords

References

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Journal for Research in Sport Rehabilitation
Volume 9, Issue 17 - Serial Number 17
February 2022
Pages 103-111

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